鸡包涵体肝炎肿瘤坏死因子α的动态变化与病变相关性的研究

Studies on the dynamic changes of tumor necrosis factor-α of chicken infected with inclusion body hepatitis virus

应用FAV_Hb株试验感染1日龄SPF雏鸡,感染后分别于第1、2、3、4、5、7、10、13、16、21、26、31、36、41、46、51、56d扑杀,采外周血和脾脏,通过微量细胞病变法经L929细胞测定其TNF浕活性,并对肝脏、脾脏、胸腺、法氏囊和盲肠扁桃体做病理学检查。结果表明FAV_Hb感染后第3d在外周血开始测出TNF浕活性,以后逐渐上升,第4d达到小的峰值,随后下降,第13d基本消失,并持续到第16d,从21dTNF浕活性又开始回升,第46d时达到最高峰,以后缓慢下降。感染鸡脾脏TNF浕活性的变化规律与外周血基本一致,感染后第2d起在脾脏测出少量TNF浕,第7d出现小峰值,以后下降到基本消失,从第21d起重新测出TNF浕,并逐渐上升,在36d到56d持续在较高的水平。病理组织学观察证实,FAV_Hb感染后,除肝脏和淋巴组织的损伤性变化外,从第1d到第56d,感染雏鸡肝脏的窦壁细胞,脾脏、胸腺、法氏囊和盲肠扁桃体的网状细胞表现出不同程度的活化增生,其增生程度与外周血和脾脏TNF浕活性基本一致?Π甯窝字琢龌邓酪蜃?浕动态变化的研究表明,FAV_Hb感染后,雏鸡体内TNF浕的消长规律与病变的发生发展有较明显的相关性。

One_day_old SPF chickens were infected with FAV_Hb strain by oral administration.They were killed after infected at first day,second day,third day,forth day,seventh day,tenth day, thirteenth day,sixteenth day,twenty one day,twenty six day,thirty six day, forty one day,forty six day,fifty one day,fifty six day,respectively.Blood and spleens were collected in order to detected the active of tumor necrosis factorα(TNFɑ) by L_(929) cells,and livers,spleens,thymus,bursas of Fabricius and the tensils of caecum were collected for the observation of pathological changes.Comparison of the control SPF chickens shown that the active of TNFɑ raised obviously in the blood and spleens of chickens by infected with FAV_Hb.The little peak appeared among third day and seventh day,and the large peak at forty sixday.The proliferation of monocytes was concord with the active of TNFɑ in the lymphical tissues of chickens infected with FAV_Hb.In addition, the pathological changes of IBH were observed in the tissues of chickens infected with FAV_Hb.