摘要
将删除33个碱基序列的CAVDNA克隆进质粒pSBII,提取质粒DNA,经过纯化、定量后作为竞争性模板,建立了一种定量检测鸡传染性贫血病毒核酸方法。该方法能够检测出的最低CAVDNA为103.5Molecules/μL,其精确度可达到100.5Molecules/μL。该方法与传统方法相比,具有节省时间、节约试剂和受其它因素干扰小等特点。
Chicken anemia virus DNA in which 33 nucleotides were deleted was cloned into plasmid pSBII.The quantificational plasmid DNA was used as competitive template.A quantitive method was established to detect CAV DNA. The minimun DNA which can detect was 10^(3.5)molecules/uL and the accuracy was 10^(0. 5)molecules/uL.It has the specialty of saving time, regents and being influenced by few factors.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2005年第3期227-229,共3页
Chinese Journal of Preventive Veterinary Medicine
基金
湖北省科技攻关重点项目(2001AA201B03)
关键词
竞争性PCR
定量检测
CAV
competitive PCR
quantification
chicken anemia virus
