内蒙古妇女宫颈癌组织中人乳头瘤病毒的PCR检测

Detection of Human Papilloma Virus by Polymerase Chain Reacti on in Cervical Cancer in Inner Mongolia

目的:了解我国宫颈癌高发区妇女宫颈癌组织中人乳头瘤病毒感染状况,探讨HPV感染与宫颈癌的关系。方法:以PCR和多重PCR法检测76例宫颈癌组织中HPV感染及型别分布状况。结果:HPV阳性率为80 . 0 %。HPV感染率在不同临床分期及不同病理分级宫颈癌组织间无显著性差异(P >0 .0 5 )。宫颈鳞癌中HPV 16阳性率为6 3 .4 % ,HPV 18阳性率为5 .6 % ,在宫颈腺癌中HPV 18的阳性率高于鳞癌,差异有显著性(P <0 . 0 5 )。结论:HPV感染是宫颈癌的主要致病因素,HPV 16和宫颈鳞癌密切相关,HPV18和宫颈腺癌密切相关,HPV感染对宫颈癌的进展及组织分化无影响。

Objective:To investigate the prevalence of human papilloma virus (HPV) in the aera with higher incidence of cervical cance r and discuss relationship between HPV infection and cervical cancer.Met hods:The HPV infection and typing in 75 cases of cervical cancer was de tected by polymerase chain reaction (PCR) and multi-PCR techniques.Resul ts:The prevalance of HPV was 80.0%,not associated with clinical stages a nd grades in cervical cancer (P>0.05).The positive rate of HPV 16 and HPV 18 in cervical squamous cancer was 63.4% and 5.6%,respectively.The p ositive rate of HPV 18 was higher in cervical adenocarcinoma than that in cervic al squamous cancer,showing significant difference (P<0.05). Conclusion:HPV infection is the major pathogenic factor for cervica l cancer.HPV 16 and HPV 18 is closely related to cervical squamous cancer and ad enocarcinoma.HPV infection has no influence in development and classification of cervical cancer.