摘要
[目的]观察沙立度胺对人乳腺癌细胞系MCF-7、MDA-MB-231细胞增殖以及血管内皮生长因子(VEGF)表达的抑制作用。[方法]应用MTT方法及半定量RT-PCR技术,分别观察沙立度胺对MCF-7和MDA-MB-231细胞增殖及VEGF表达的抑制作用。[结果]沙立度胺在(10～100)μg/ml范围内对MCF-7和MDA-MB-231细胞有明显的抑制增殖作用,并伴随VEGFmRNA表达水平下降;50μg/ml明显抑制两株细胞VEGFmRNA的表达。[结论]沙立度胺在一定浓度范围内能抑制乳腺癌细胞VEGFmRNA的表达,抑制细胞增殖反应。
To assess the effects of thalidomide on cell proliferation of MCF-7, MDA-MB-231 and VEGFmRNA level in human breast cancer cell lines. Breast cancer cells were treated with thalidomide for 24h^72h, and cell proliferation was examined by the method of MTT. Half fixed quantitation RT-PCR technique was applied to distinguish the level of VEGFmRNA expression. Within the concentration of 10~100μg/ml, thalidomide strongly inhibited proliferation of MCF-7 and MDA-MB-231 in breast cancer cells, the growth inhibitory effect on the cell was accompanied by a decrease in VEGFmRNA level. When the drug is 50μg/ml, thalidomide strongly inhibited expression of VEGFmRNA. [Conclusion] Within a certain durg concentration, thalidomide can inhibite breast cancer cell proliferation and expression of VEGFmRNA.
出处
《肿瘤学杂志》
CAS
2005年第1期26-28,共3页
Journal of Chinese Oncology
关键词
乳腺肿瘤
沙屯度胺
内皮生长因子
细胞系
breast neoplasms
thalidomide
endothelial growth factor
cell lines
