黄芪多糖对IL－2／LAK抗肿瘤增强作用的动物实验研究

STUDY OF THE ANTITUMOR EFFECT WITH ASTRAGALUS POLYSACCHARIDE AND IL-2/_LAK

采用Ｃ５７ＢＬ／６鼠荷其自发产生的黑色素瘤Ｂ＿１６细胞，以生存期为指标，建立了黄茂多糖（ＡＰＳ）增强ＩＬ－２／ＬＡＫ抗肿瘤作用的动物模型，结果表明：ＡＰＳ自身具有一定的抗肿瘤作用，ＡＰＳ（５ｍｇ／ｋｇ）与ＩＬ－２／ＬＡＫ（５×１０￣３Ｕ／ｋｇ、５×１０￣７／ｋｇ）的抗肿瘤作用相似，荷瘤鼠生存期分别为２１．５７±１．８１天和２０．８６±１．８６天（荷瘤对照鼠为１５．７１±０．４９天）；ＡＰＳ对ＩＬ－２／ＬＡＫ的抗肿瘤效应有明显的增强作用，二者在上述剂量下联合应用，荷瘤鼠生存期为２４．８６±２．７３天（Ｐ＜０．０１）。并对荷瘤鼠进行动态细胞免疫功能观察，提示：ＡＰＳ和ＩＬ－２／ＬＡＭ均具有抵抗鼠脾ＮＫ细胞活性，ＩＬ－２产生能力和腹腔Ｍψ吞噬能力下降的作用，ＡＰＳ对ＩＬ－２／ＬＡＫ仍有显著增强效应。

e established the experimental animal model using the mouse C_57BL/6 which carried themelanocytoma B_16 cells, The life span data of Astragalus Polysaccharide(APS)therapy and thecombined therapy with IL-2 /LAK were collected.The resuIts showed that APS alone has anti-tumor effect :the APS therapy(5mg/kg)is compared favourably the combination of IL-2(5×10￣3U/kg)with LAK(5× 10￣7U/kg).The life spans of the tumor carrying mice were 21.57±1。81 and 20.86±1.86 days respectively(control group:15.71±0.49 days).APS enhanced sig-nificantly the IL-2/LAK antitumor effect.The combined therapy medicines at the abovedosages were given to the tumor carrying mice and the life span is 24.86±2.73 days(P < 0.01).The dynamic observation of cellular immune function was made in the treatment process oftumor carrying mice and the resuIts show that the decrease tendency of NK activity,IL-2 prc-ducing ability of spleen cells and abdominal Mψ phagocytatic function with the time were antago-nized by the APS,IL-2/LAK,and the combined therapy was preffered。