青天葵挥发油化学成分分析

Analysis of the Constituents of Volatile Oil from Nervilla fordii by Gas Chromatography - Mass Spectrometry

[目的]分析青天葵挥发油的化学成分。[方法]采用水蒸汽蒸馏法提取青天葵挥发油,运用气相色谱一质谱联用(GC-MS)技术和标准图谱检索对照、核对及补充检查的方法,分离和鉴定挥发油中各化学组分,用离子流色谱峰面积归一法计算各化学组分的相对含量。[结果]从青天葵挥发油中分离出89个化学组分峰,鉴定出53个峰(占挥发油总含量的91.82%)。[结论]青天葵挥发油的主要化学成分为:6,10,14-三甲基2-十五烷酮(13.55%),4-乙烷基-顺-3-硫代环[4,4,0]癸烷(6.54%),4-甲基-N-(2-氧络-2苯乙基)苯磺酰胺(6.33%),植醇(6.32%),δ-Cadinol (4.54%),β-紫罗兰酮(4.43%),石竹烯氧化物(4.13%),它们的含量占挥发油总含量的45.84%。

[Objective] To analyze the constituents of volatile oil from Nervilla fordii (NF) by gas chromatography-mass spectrometry (GM-MS). [Methods] Volatile oil was extracted from NF by steam distillation and the constituents of volatile oil were isolated and identified by GM-MS. The relative contents of the volatile constituents were calculated by ionic flow chromatographic area normalization method. [Results] Eighty-nine components were separated. Among them, fifty-three components were identified, accounting 91.82% of the total volatile oil. [Conclusion] The main constituents identified from NF were: 2-pentadecanone, 6,10,14-trimethyl (13.55%), 4-ethyl-trans-3-thiobicyclo [4,4,0] decane (6.54%), benzenesulfonamide, 4-methyl-N- (2-oxo-2 phenylethyl) (6.33%), phytol (6.32%),δ-carinol (4.54%), β-ionone (4.43%), caryophyllene oxide (4.13%). The amount of the above constituents accounts 45.84% of the total volatile oil.