康欣胶囊及其拆方对肾虚血瘀型血管性痴呆大鼠模型性激素雌二醇(E2)、睾酮(T)及E2/T比值的影响

The Study on Decoction of KANGXIN Capsule and Its Minor Components are Influenced on E2、T and E2/T Ratio of Kidney Asthenia with Blood Stasis Vascular Dementia Rat Model

目的为观察康欣胶囊及其拆方对(VD)模型(肾虚血瘀型)大鼠外周血E2、T、E2/T的影响。方法采用电灼烧封闭双侧椎动脉和摘除单侧性器官法制作(VD)模型(肾虚血瘀型)大鼠,用康欣胶囊全方及其拆方和阳性西药喜得镇给药1个月,电镜、光镜观察各组下丘脑神经细胞情况,用DFM-96型多管放射免疫记数器检测大鼠外周血E2、T、E2/T。结果电镜观察各组模型大鼠下丘脑出现不同程度的线粒体水肿,嵴断裂,基质变浅,核染色质固缩及线粒体空泡变性的变化,空白对照组下丘脑嗜银染色可见神经纤维缠结;外周血雌雄激素水平:雌性康欣胶囊全方、补肾、健脾、养血活血、西药组E2与雌性空白组比较低(P<0.01);雄性康欣胶囊全方和补肾组E2与空白对照雄性组比较低(P<0.05)雌性康欣胶囊全方、健脾组的E2/T比值低于雌性养血活血组(P<0.01);雌性补肾组E2/T比值低于雌性养血活血组(P<0.05);雌性康欣胶囊全方、健脾组E2/T比值低于雌性空白组(P<0.01);雌性康欣胶囊全方、健脾组的E2/T比值低于西药组(P<0.05),康欣胶囊全方、健脾、补肾能有效的调节雌性E2水平,使高水平E2降低和调节、降低肾虚血瘀型血管性痴呆模型大鼠E2/T比值;全方、补肾组能有效降低雄性模型大鼠E2水平。结论康欣胶囊全方、补肾。

OBJECTIVE Observed decoction of KANGXIN capsule and its minor components were influenced on E2, T, E2/T ratio of kidney asthenia with blood stasis vascular dementia rat model. METHODS Used electric iron to burn and block both side vertebral arteries and amputated one side genital organ(testis or ovary) of rat, made kidney asthenia with blood stasis of VD rat model. KANGXIN capsule complete component and its minor components; and Dihydroergotaxine had been given for 1 month; at the same time, established control model group (hadn't given any medication), complete component, kidney tonic, pancreas tonic, blood tonic with promoting blood circulation, and western medicine Dihydroergotaxine groups. Used electron microscopy and light microscopy to observe nerve cells of hypothalamus of each group; used Radio Immunoassay (DFM-96) to analyze E2, T, E2/T of model rat.RESULTS Under electron microscopy: mitochondria of hypothalamus of each group were appeared slightly swelling and microstructures of mitochondrion were damaged in different scale. Under light microscopy, silver stain slides of hypothalamus of control model group were observed some nerve fiber tangle. E2 of female of complete component, kidney tonic, pancreas tonic, blood tonic with promoting blood circulation, western medicine groups were lower than female control groups (P<0.01). E2 of male complete component and kidney tonic group was lower than male control group. E2/T ratio of female complete component, pancreas tonic were decrease compared with female blood tonic with promoting blood circulation group (P<0.01); E2/T ratio of female kidney tonic group was decrease than female blood tonic with promoting blood circulation group (P<0.05); E2/T of female complete component and pancreas tonic groups were decrease compared with female control group (P<0.01); E2/T of complete component and pancreas tonic group were lower than western medicine group (P<0.05).CONCLUSION Decoction of Kangxin capsule complete component, pancreas tonic and kidney tonic component regulated effectively female estradiol those decreased higher level of female estradiol, regulated and decreased E2/T ratio of kidney asthenia with blood stasis VD rat model. Complete component and kidney tonic component decreased effectively E2 of male model VD rat. In short, Kangxin capsule complete component and kidney tonic component were able to decrease E2 of female and male VD model rat, let E2/T ratio become physiology balance level. Otherwise, Morris Swimming Pool Test showed Kangxin capsule complete component and kidney tonic increased effectively swimming ability and times of Spatial Probe Test, decreased duration of Escape Latency (second).