肝素、三氟啦嗪对去甲肾上腺素调控大鼠腹腔巨噬细胞Ia抗原表达

BLOCKING OF THE PROMOTIVE EFFECT OF NORE-PINEPHRINE ON THE IA ANTIGEN EXPRESSION OF RAT PERI-TONEALMACROPHAGES BY HEPARIN ANDTRWLU0PER.AZINE

用细胞膜放射性标记和离子交换层析法、Ｃａ￣（２＋）指示剂的分光光谱法和ＡＰＡＡＰ桥联酶标法分别检测Ｍ内ＩＰ＿３、〔Ｃａ￣（２＋）〕ｉ和膜上Ｉａ抗原的表达，研究ＩＰ＿３受体阻断剂肝素（Ｈ）和钙调蛋白（ＣａＲＰ）抑制剂三氟啦嗪（ＴＦＰ）对ＮＥ调控ＭＩａ抗原表达的影响。结果显示：Ｈ（４０μｇ／ｍｌ）不影响ＮＥ（１０￣（－８）ｍｏｌ／Ｌ）增高Ｍ内ＩＰ＿３的作用（４５２±３０ｃ．ｐ．ｍ．／１０￣６ｃｅｌｌｓ，ＮＥ组：４４２±２２ｃ．ｐ．ｍ．／１０￣６ｃｅｌｌｓ，Ｐ＞０．０５）；但能阻断ＮＥ增高Ｍ〔Ｃａ￣（２＋）〕ｉ的作用（１１４±４３ｎｍｏｌ／Ｌ，ＮＥ组：３２２±７６ｎｍｏｌ／Ｌ，Ｐ＜０．０１）；还能阻断ＮＥ促进ＭＩ－Ａ、Ｉ－Ｅ表达的效应（４６％±４％、４５％±７％，ＮＥ组：６４％±８％、５８％±６％，Ｐ＜０．０１）。ＴＦＰ（５０μｍｏｌ／Ｌ）不影响ＮＥ升高Ｍ〔Ｃａ￣（２＋）〕的作用（３２８±８９ｎｍｏｌ／Ｌ，ＮＥ组：３２２±７６ｎｍｏｌ／Ｌ，Ｐ＞０．０５），却能阻断ＮＥ促进ＭＩ－Ａ、Ｉ－Ｅ表达的效应（４５％±４％、４４％±５％，ＮＥ组：６４％±８％、５８％±６％，Ｐ＜０．０１）。结果提示：ＮＥ调控ＭＩａ抗原表达的效?

ell membrane isotope labelling and ion-exchange chromatography were used to determinethe IP_3 conten*t, Fura-2 was used as a fluorescence indicator to assav the intracellular calciumion concentration( Ca￣(2+) i),and alkaline phosphatase anti-alkaline phosphatase(APAAP ) en-zyme immunoassay was used to detect the Ia antigen expression of rat peritoneal macrophages(MΦs). It was found that the effect of norepinephrine(NE,10￣(-8) nol/L,the same below) to in-crease Ca￣(2+) i and to promote Ia antigen expression of the MΦs could be blocked by heparin(H,40μg/ml, the same below), an inositol triphosphate(IP_3) receptor blocking agent, despite thatthe latter did not interfere with the effect of NE to increase the IP_3 content of the MΦs.Triflu-operazine(TFP, 50μmol/L), an antagonist of the calcium regulatory protein (CaRP),could alsoblock the effect of NE to promote Ia antigen expression,without influencing the effect of NE toincrease Ca￣(2+) i of the MΦs. These findings suggest that the promotive effect of NE of Ia anti-gen expression of the MΦs is dependent on the calcium ions released from calcium pool sensitiveto IP_3 stimulation and that binding of Ca￣(2+) with CaRP is an indispensable step of the NE effectto promote Ia antigen expression on the MΦs.