白介素2和血小板活化因子对嗜酸性粒细胞的趋化作用比较

COMPARISON OF CHEMOTAXIS OF rhIL-2 AND PLATELET-ACTIV ATING FACTOR FOR EOSINOPHILS

用改良Ｂｏｙｄｅｎ法，测定重组人白介素２（ｒｈＩＬ－２）和血小板活化因子（ＰＡＦ）对豚鼠嗜酸性粒细胞（Ｅｏｓ）和中性粒细胞（Ｎｅｕ）的趋化和化学激动作用，ｒｈＩＬ－２对Ｅｏｓ的最大趋化作用浓度为１０￣（－１２）ｍｏｌ／Ｌ，较ＰＡＦ的作用强约１００００倍，１０￣（－５）～１０￣（－９）ｍｏｌ／Ｌ范围内，ＰＡＦ对Ｅｏｓ和Ｎｅｕ有相似的趋化作用．但在对Ｅｏｓ呈趋化作用的１０￣－９～１０￣－１３ｍｏ１／Ｌ范围内。ｒｈＩＬ－２对Ｎｅｕ无明显作用．另外，１０￣（－１２）ｍｏｌ／Ｌ的ｒｈＩＬ－２对Ｅｏｓ的趋化和化学激动作用无显著差异（Ｐ＞０．０５），而１０￣（－８）ｍｏｌ／Ｌ的ＰＡＦ对Ｅｏｓ的趋化作用则显著大于化学激动作用（Ｐ＜０．０５）。以上结果表明，ＩＬ－２是一种极强的选择性Ｅｏｓ趋化介质．

he chemotaxis and chemokinese of rhIL-2 and platelet-activating factor(PAF)foreosinophils and neutrophils from guinea pigs were determined.The maximal chemotactic ef-fect of rhIL-2 for eosinophils was at 10￣(-12) mol/L,which was 10000 times of the effect ofPAF. Nevertheless, neutrophils did not show significant migration response to rhIL-2 of10￣(-9)～10￣(-13)mol/L. In contrast,PAF in concentration of 10￣(-5)～10￣(-9)mol/L had similar chemo-taxis for eosinophils. There was no significant difference of chemotaxis and chemokinese ofrhIL-2 for eosinophils(P>0.05).However,chemotaxis of PAF for eosinophils was signifi-cant higher than chemokinese(P<0.05). The result showed that IL-2 was one of the mostpotent selective chemoattractant for eosinophils.