烧伤后血清触珠蛋白对T淋巴细胞功能的影响

EFFECT OF SERUM HANPTOGLOBIN ON TA-LYMPHOCYTEFUNCTION FOLLOWING BURNS

采用亲和层析法从烧伤血清纯化触珠蛋白（Ｈａｐｔｏｇｌｏｂｉｎ，ＨＰ）后，体外观察了纯化ＨＰ对正常小鼠胸腺细胞在ｃｏｎＡ刺激下增殖与ＩＬ－２产生及对ＩＬ－２诱导的ＩＬ－２依赖细胞株（ＣＴＬＬ－２）增殖的影响。结果表明，纯化ＨＰ在相当于烧伤血清ＨＰ浓度时，对小鼠胸腺细胞增殖、ＩＬ－２产生及ＣＴＬＬ－２增殖具有非常明显的抑制作用；但在相当于正常血清ＨＰ浓度时纯化ＨＰ则无抑制作用。提示烧伤血清高浓度的ＨＰ可能是烧伤后Ｔ淋巴细胞免疫功能低下的一个重要原因。

his study was imtended to investigate the effect of hgptoglobin,purified from the serum ofburned patients by affinity chromatography,on the proliferation and interleukin-2(IL-2) pro-duction of the normal murine thymocytes stimulated by ConA and the proliferation of IL-2 de-pendent cell line(CTLL-2)induced by recombinant human IL-2 so as to clarity the role ofhatoglobin in the postburn T-lymphocyte dysfunction.The results showed that purified hapto-golobin,atthe level epuivalent to the concentration found in serum of burned patients,signifi-cantly inhibited the proliferation,and IL-2 production of thymocytes and CTLL-2 proliteration,but then it exhibited no immunosuppressive effects at the level equivalent to the concentrationfound in serum of normal volunteers,From these results,it is suggested that extraordinary in-crease in haptoglobin levels may be an important factor of impaired T-lymphocyte responses fol-lowimg burns.