前列腺特异性抗原（PSA）的ABC－ELISA建立和临床初步应用

ENZYME IMMUNOASSAYS FOR THE DETECTION OF PROSTATE一SPECIFIC ANTIGEN UTILIZNG BIOTIN/AVIDIN SYSTEM ANDCLINICAL PRELIMINARY APPLICATION

前列腺特异性抗原是从人精液中提取的一种糖蛋白。血清中ＰＳＡ测定对前列腺良、恶性疾病有重要诊断价值。利用生物素─—抗生物素系统与ＥＬＩＳＡ技术相结合，建立了测定血清中ＰＳＡ含量的ＡＢＣ－ＥＬＩＳＡ竞争法。此法较常规的ＥＬＩＳＡ敏感度高，较放免法安全、经济。本文对其方法的灵敏度、重复性、可靠性进行了探讨。测得正常男性血清ＰＳＡ范围是０～３．３ｎｇ／ｍｌ（ｎ＝８６），女性血清ＰＳＡ未测出（ｎ＝１５）。

SA is a glycoprotein whic is extracted from human sperm.The lever of PSA in serum issignificant on dignosing prostate disease. The biotin/avidin system was incorporated into the en-zyme一linked immunosorbent assay(ELISA)technigue(ABC一一ELISA)for the detection ofprostate specific antigen(PSA)in serum.The assay was reprodu cible as within and between as-say yielded a colnfficient of variation of 4.9%～5.23%and 6.26%～8.21%.PSA was not de-tectable in sera from normal femal(n=15).of 1.02±1.44ng/ml(ranging from was lessthan 0.5 to 2.8)was obtain from 86 normal mates.The ABCELISA is more sensitive than otherstandard ELISA.