摘要
用Fura-2作为荧光探针测定大鼠腹腔巨噬细胞(M)内钙离子浓度([Ca ̄(2+)]i),APAAP桥联酶标法检测M表面Ia抗原的表达。结果表明:5×10 ̄(-6)mol/L的乙酞胆碱(Ach)可使M[Ca ̄(2+)]i;明显上升,可促进M表面I-A和I-E抗原的表达,而阿托品(10 ̄(-5)mol/L)可阻断Ach升高[Ca ̄(2+)]i的作用。阿托品、三氟啦嚎(TFP,50μmol/L)、EGTA(6mmol/L)均可阻断M促进MIa抗原表达的作用,cAMP依赖性蛋白激酶抑制剂(PKI,25μg/ml)对Ach促进MIa抗原表达的作用无影响。
ura-2 was used as fluorescent indicator to measure intra-cellular Ca ̄(2+) concentration[Ca ̄(2+)]i and APAAP enzyme immunoassay to detect Ia antigen expression of rat peritonealmacrophages. The results showed that acetylcholine(Ach,5×10 ̄6mol/L)could increase[Ca ̄(2+)]iand promote Ia antigen expression.If macrophages had been pretreated with atropine; EGTA,trifluoperazine,respectively,the effect of Ach to promote Ia antigen expression was blocked.Theeffect of Ach to increase[Ca ̄(2+)]i was also blocked by atropine. Pretreatment with PKI could notinhibit the effect of Ach to promote Ia antigen expression.
出处
《中国免疫学杂志》
CSCD
北大核心
1994年第3期159-160,165,共3页
Chinese Journal of Immunology
基金
国家自然科学基金
关键词
乙酰胆碱
钙离子
IA抗原
巨噬细胞
Acetylcholine Macrophage Calciumion Ia antigen Intracelluar