ATRA与细胞因子对早幼粒白血病细胞体外增殖的影响

EFFECTION OF ATRA AND CYTOKINES ON PROLIFERATIONOF PRIMAKY APL CELLS

原代早幼粒白血病（ＡＰＬ）细胞在体外增殖试验中，ｒｈＧ－ＣＳＦ或ｒｈＴＮＦ均呈现增殖抑制作用，ｒｈＧＭ－ＣＳＦ或ＩＬ－６无明显抑制其增殖作用，且在一定浓度范围内呈增殖促进作用，本试验浓度条件下，单独ＡＴＲＡ对原代ＡＰＬ细胞表现为对增殖有抑制作用，对正常造血细胞无明显增殖促进或抑制作用，较低浓度ＡＴＲＡ合并上述４种因子对原代ＡＰＬ细胞都表现为增殖抑制作用，其中ｒｈＧＭ－ＣＳＦ或ＩＬ－６合并ＡＴＲＡ比单独ＡＴＲＡ对ＡＰＬ细胞增殖的抑制作用小。

he results showed that proliferation of primary acute promyelocytic leukemia(APL)cellscould be inhibited by rhG-CSF or rhTNF.Inhibition of proliferation was not achieved in experi-ment of rhGM-CSF or IL-6 on primary APL cells,at some density they could promote prolifera-tion of APL cells,ATRA alone could inhibit proliferation of primary APL cells under this exper-iment conditions, It was also observed that ATRA alone didn′t obviously inhibit or promotegrowth of normal hematopoietic cells. The results suggested that hyperleukocytosis couldn′t con-tribute to direct effect of ATRA in vitro,the mechanism of the hyperleukocytosis needs shouldbe further detected.