MHC－I类分子在诱导肿瘤浸润淋巴细胞细胞毒活性中的作用

THEROLEOFMHC-1MOLECULESININDUCTIONOFSECIFICCYTOTOXICACTIVITYOFTILs

选用Ｈ＿２２（小鼠肝癌）、Ｂ＿１６（小鼠黑色素瘤）和Ｅ＿（１０）（小鼠淋巴瘤）三种动物模型分离肿瘤浸润淋巴细胞（ＴｕｍｏｒｉｎｆｉｌｔｒａｔｉｎｇＬｙｍｐｈｏｃｙｔｅｓ，ＴＩＬｓ），在含ＩＬ－２２００Ｕ／ｍｌ的培养系统中以６０钴灭活自身瘤细胞，周期性刺激进行扩增。实验发现，被选用的三种动物瘤株中。仅从Ｅ＿（１０）淋巴瘤分离的ＴＩＬ显示明显的增殖和对自身瘤细胞的杀伤活性。对三种瘤株体外ＭＨＣ限制性观察结果表明，未测出Ｈ＿（２２）、Ｂ＿（１６）瘤株细胞表达Ｋ￣ｂ、Ｄ￣ｂ、Ｋ￣ｋ、Ｄ￣ｋ、Ｋ￣ｄ、Ｄ￣ｄ位点编码的ＭＨＣ－Ｉ类分子，而Ｅ＿（１０）瘤细胞则表达强阳性的Ｋ￣ｂ、Ｄ￣ｂ位点编码的ＭＨＣ－Ｉ类分子。此外，Ｈ＿（２２）、Ｂ＿（１６）瘤细胞可在多种品系（Ｃ＿（５７）ＢＬ／６、Ｃ＿３Ｈ、Ｂａｌｂ／Ｃ及昆明种）小鼠中生长，而Ｅ＿（１０）瘤株只限制在Ｃ＿（５７）ＢＬ／６品系小鼠中生长。

_(22)(Hepatoma),B_(16)(Melanoma)and E_(10)(Lymphoma)were used to separate tumor infiltrat-ing lymphocytes(TILs). TILs were cultured in RPMI1640 Medium Containing IL-200U/mland periodically stimulated with inactivated autologous tumor cells.TILs with specific cytotoxici- ty from E_(10)(lymphoma) but not H_(22)、B_(16) could be induced successfully.Experiments in whichH_(22)、B_(16) and E_(10) tumor cells were separately innoculated subcutaneously in several strains of mice(C_(57)BL/6、 C_3H、Balb/C and Kun-ming strain)showed that both H_(22) and B_(16)tumor cells(couldgrow in all strains of mice,but E_(10) tumor was seen only in C_(57)BL/6mice. Using one-way mixedlymphocyte reaction to induce allo-specific cytotoxic T cells,we found that allo-specific CTLs a- gainst H-2￣b haplotype could kill the E_(10) but not the B_(16) cells, Again, the allo-specific CTL againstH-2￣k haplotype showed no cytotoxicity to H_(22)tumor cells.Furthermore.Using antiH-2K. D spe-cific typing serum to detect the expression of MHC-1molecules on the surface of tumor cells,wefound that MHC-1molecules could not be detected on the surface of H_(22)and B_(16) tumor cells butdetected on E_(10)lymphoma cells.This implied that tumor cells without expression or only withlow expression of MHC-1molecules could not be used to induce the specific cytotoxicity of tu- mor infiltrating lymphocytes.