应用共聚焦激光扫描显微镜对脑脊液淋巴白血病细胞线粒体的研究

Study of mitochondria in cerebrospinal fluid leukemia cells by CLSM

目的通过共聚焦激光扫描显微镜(CLSM)对脑脊液淋巴白血病细胞线粒体进行立体、动态、定量研究。方法将所观察的脑脊液细胞分为正常淋巴细胞组、急性淋巴白血病细胞组、鞘膜内注射治疗后急性淋巴白血病细胞组。以线粒体荧光探针MitotrackerGreenFM标记后,得到线粒体的断层扫描图像、荧光强度地形立体分布图定量测量线粒体的荧光强度值。结果急性淋巴白血病细胞(357.13+60.99)与正常淋巴细胞(269.38+56.11)、鞘膜内注射治疗后淋巴白血病细胞(317.41+57.57)相比,平均荧光值有非常显著性差异(P<0.01),线粒体空间立体分布有明显差别。结论采用CLSM对中枢神经系统淋巴白血病的诊断、鉴别诊断、细胞分化程度和功能状态判断有一定意义。

Objective To three-dimensionally, dynamically and quantitatively investigate the mitochondria of cerebrospinal fluid leukemia cells by confocal laser scanning microscope(CLSM). Methods Mitochondria of all lymphocytes(normal lymphocytes, leukemia cells, cured leukemia cells)were stained with fluorescent probe Mitotracker Green FM, sectional scanograph and fluorescent intensity stereophotography were obtained and finally fluorescent values of cellular mitochondria were measured individually. Results Compared with normal lymphocyte cells(269.38 + 56.11), of the three-dimensional character mitochondria of leukemia cells varied and the fluorescent value(357.13 + 60.99) differences were statistically significant (P<0.01). Compared with cured leukemia cells(317.41 + 57.57), of the three-dimensional character mitochondria of leukemia cells varied and fluorescent value differences were statistically significant too (P < 0.01). Conclusions The experimental works mentioned above are feasible, and can demonstrate the difference of mitochondria between cerebrospinal fluid normal lymphocytes and leukemia cells three-dimensionally, dynamically and quantitatively. This is useful for central nervous system leukemia diagnosis, differential diagnosis, and the evaluation of cell differentiation level and function.