淋巴因子激活的骨髓细胞对混合淋巴细胞培养的抑制作用

SUPPRESSION OF THE MIXED LYMPHOCYTE RESPONSE BYLYMPHOKINE-ACTIVATED BONE MARROW CELLS

采用单向混合淋巴细胞培养（ＭＬＣ）方法，从不同主要组织相容性抗原（Ｈ－２）单型小鼠的多种组合中，验证淋巴因子激话的骨髓细胞（ＬＡＢＭＣ）的Ｖｅｔｏ效应。结果表明：ＬＡＢＭＣ于培养当天加入单向ＭＬＣ，在与反应细胞低比例时可特异住抑制与其带有相同Ｈ－２单型的刺激细胞参与组合的增殖应谷和杀伤效应的诱导；在与反应细胞高比例时其抑制不具特异住，但对与其带有相同Ｈ－２单型的刺激细胞参与组合的抑制较强，提示ＬＡＢＭＣ在低比例时具Ｖｅｔｏ活性，在高比例时同时具Ｖｅｔｏ和自然抑制（ＮＳ）活性。ＬＡＢＭｃ于培养第二天加入单向ＭＬＣ则不显示抑制效应，提示ＬＡＢＭＣ对ＭＬＣ的抑制具有时相效应，流式细胞术（ＦＣＭ）显示ＬＡＢＭＣ中ｌｙｔ－２（＋）细胞成分明显增加．

MLR-CML model was established successfully by using oneway MLC technique in a va-riety of experimental combinations of inbred strains with different H-2 haplotypes to observe theveto activity of LABMC. Results showed that:(1)LABMC added to MLC on day 0 specificallyinhibited the proliferation response and cytotoxic effect in MLC in which stimulator cells havethe same H-2 haplotypes with LABMC at the LABMC/responder cell ratio of 1/10, but non-specifically suppressed the proliferation response and cytotoxic effect in MLC in vvhich stimulatorcells have the same or different H-2 haplotypes with LABMC at the higher LABMC/respondercell ratios of 1/3,1/1 and 3/1.Suggesting that LABMC have veto activity at the lower ratios,but have veto and natural suppressor(NS)activity at higher ratios.(2)LABMC added to MLCon day 2 had no suppressive activity. suggesting that the suppression of LABMC on MLC is re-lated to phase, (3)Phenotype analysis by flow cytotnetry showed that lyt-2(+)cell increased inLABMC。