同种动物间TSH抗独特型抗体制备及和AITD关系

PREPARATION OF TSH ANTI-IDIOTYPIC ANTIBODY IN HOMO-GENEOUS ANIMAL AND RELATIONSHIPTO AITD

根据自身免疫性甲状腺疾病（Ａｕｔｏｉｍｍｕｎｏｔｈｙｒｏｉｄｄｉｓｅａｓｅ，ＡＩＴＤ）发病机制新学说：独特型－抗独特型免疫网络学说，用兔抗人ＴＳＨ抗体在同种家兔中制备了ＴＳＨ抗独特型抗体（ＴＳＨＡｂ＿２），并从免疫学、生物学功能方面进行了鉴定，同时用豚鼠脂肪中ＴＳＨ受体纯化了ＴＳＨＡｂ＿２并检测了兔血清Ｔ＿４、ＴｍＡｂ的值，发现免疫后两者均明显升高（Ｐ＜０．０１）．本实验支持ＡＩＴＤ中独特型－抗独特型免疫网络学说。

abbit anti-hTSH anti-idiotypic antisera were raised by immunization with rabbit anti-hTSH antibody。 Meanwhile,we determinate the raised TSHAb_2,and purify the TSHAb_2 withTSH receptor T_4 and TmAb increase significantly after the rabbit was immunized(P<0.01)··This test surpports the hypothesis of idiotypic immunonet of AITD;