人胎脾抗癌效应细胞及相关细胞因子的胚胎发育动力学研究

STUDIES ON THE ONTOGENETIC KINETICS OF HUMAN ANTI-UMOR EFFECTOR CELLS AND RELATIVE CYTOKINES FROMFETAL SPLEEN

对不同胎龄胎脾抗癌效应细胞及相关细胞因子的胚胎发育动力学进行了系统观测。结果显示：２０周龄前基本无ＮＫ活性和Ｍ杀伤功能，但可随胎龄增长而逐渐加强，至出生前后已接近成人水平，ＬＡＫ活性在１６周龄即与成人基本相同。ＩＬ－２，ｒＦＮ－α和ＩＬ－６能分别促进ＮＫ和ＬＡＫ活性。胎脾Ｍ２０周龄即合成高水平ＩＬ－６，出生前已显著高于成人，３２周龄时分泌ＩＬ－１的能力达成人水平，ＴＮＦ活性达高峰。ＦＳＭＣ合成ＩＬ－２的能力在３６周龄才达成人水平，合成的ＩＬ－６至出生前仍低于成人。ＦＳＭＣ的ＨＬＡ－ＤＲ抗原表达显著低于成人。因此以胎脾细胞作为抗癌效应细胞来源是安全、有效、可行的。

he ontogenetic kinetics of anti-tumor effector cells and related cytokines from fetal spleenof different ages were studied systematically in this paper,The result is as follows. Before 20GW,no activity of NK and Mrp could be assayed.But it could increase with the fetal age. Tillbirth was the cytotoxicity of M close to that of adult. In contrast,the activity of LAK was al-ready about the same level as that of adult at 16 GW. Mrp from 20-GW-fetal spleen could pro-duce IL-6 at high level;At 32 GW,it could secret the same level of IL-1 as that of adult.Butthe eprodtict of TNF at same GW reached the high level only. On the contrary,only at 36 GWcould FSMC produce the same level of IL-2 as that of adult and the level of IL-6 was still lowerthaii that of adult until birth.The expression of HLA-DR antigen of FSMC was significantlylower than that of adult.The studies provide not only new data for immunobiology but also the-oretical basis for the application of anti-tumor effector cells.