多重聚合酶链反应系统快速诊断结核和其他细菌感染

Rapid Diagnosis of Tuberculosis and Other Bacterial Infections by Multiplex Polymerase Chain Reaction

目的探讨多重聚合酶链反应(PCR)系统用于诊断细菌感染,并区分结核和其他细菌感染。方法多重PCR系统检测129份临床样本包括脑脊液108份、胸水10份、腹水11份,与培养结果比较,分别评估该系统检测普通细菌和结核杆菌敏感性和特异性。结果本系统检测临床样本中普通细菌和结核杆菌的敏感性均达100%,特异性分别为86%和81%。结论多重PCR系统可成功地用于临床快速诊断细菌感染,并有效区分结核和其他细菌感染。

Objective Multiplex polymerase chain reaction(PCR) was utilized to diagnose bacterial infections and to differentiate tuberculosis from other bacterial infections. Methods One hundred and twenty nine clinical specimens including cerebrospinal fluid (n = 108),pleural fluid( m = 10), peritoneal fluid(n = 11), were tested by this assay, and compared with the results of bacterial culture for assessing the sensitivity and specificity of this system in detecting bacteria and tubercule bacilli respectively. Results This multiplex PC'R system showed that sensitivity reached 100% on detecting both general bacteria and tubercule bacilli, and specificity was 86% on examining general bacteria and 81% on determining tubercule bacilli. Conclusion This multiplex PCR system is a rapid and reliable method for diagnosing bacterial infections and differentiating tuberculosis from other bacterial infections.