CytosporoneB的分离纯化,结构鉴定及其生物活性的初步研究

Purification,Elucidation and Activities Study of Cytosporone B

从福建九龙江口红树林自然保护区桐花树树皮中分离出内生真菌HTF3菌株,用柱层析和正相HPLC的方法从该菌株的发酵液中纯化出一单体化合物,经1D- 、2D -NMR和ESI -MS等方法鉴定该化合物为CytosporoneB.活性检测发现它具有较强的抗肿瘤活性和较广谱的抗真菌活性,本文报道这类物质有抗肿瘤活性,作用机理值得进一步深入研究.

The microorgamism is an attractive resource for chemically interesting and biologically significantly secondary metabolites.Mangroves are special woody plant communities in the intertidal zone of tropical and subtropical coasts.They are proved to be natural microorganisms and new metabolites storage.An endophytic fungus,Dothiorella sp.,strain HTF3 was isolated from mangrove plant Avicennia marina at the estuary of Jiulong River,Fujian Province.Its fermentation broth was extracted with ethyl acetate and the extract was subjected to column chromatography on silica gel and HPLC.A compound was purified,its structure was elucidated by ESI,1D- and 2D-NMR spectra as Cytosporone B.Its IC_(50) values were 4 μg/mL and 0.062 5 μg/mL against human epidermal carcinoma of oral cavity,KB cell line and human Burkitt’s lymphoma,Raji cell line,respectively.Furthermore,it showed broad activities against fungi,the MIC against Aspergillus niger、Trichoderma sp.and Fusarium sp were 0.125 mg/mL、62.5 μg/mL and 62.5 μg/mL,respectively.Its high antitumor activities and broad anti-fungi spectra indicates that it is worthy further research.