芦荟多糖对体外培养人表皮细胞分泌细胞因子及一氧化氮的影响

Effect of aloe vera polysaccharide on the release of cytokines and nitric oxide in cultured human keratinocytes

目的研究芦荟多糖对体外培养人表皮细胞分泌细胞因子及一氧化氮(NO)的影响。方法测定经25、50、100、200和400mg/L,不同浓度芦荟多糖作用后的人表皮细胞培养上清液中转化生长因子-α(TGF-α)、TGF-β、白细胞介素-1β(IL-1β)、IL-6、IL-8、肿瘤坏死因子(TNF)及NO的水平;对照组则用等体积的细胞培养液处理。结果与对照组比较,经芦荟多糖作用后,培养液中TGF-α、TGF-β1、IL-1β、IL-6、IL-8和TNF水平呈不同程度升高,其差异有显著性(P<0.05或P<0.01);且随着芦荟多糖作用浓度的增加,其效应与剂量明显相关(P<0.01);而NO水平与对照组比较呈显著性下降(P<0.01),量-效关系明显(P<0.01)。结论芦荟多糖促进人表皮细胞分泌TGF-α、TGF-β1、IL-1β、IL-6、IL-8及TNF,而对NO释放则具有抑制作用。

Objective To investigate the effects of polysaccharide extracted from Aloe Barbadensis on the release of cytokines and nitric oxide (NO) in cultured human keratinocytes. Methods The levels of transforming growth factor-α(TGF-α), TGF-β1, interleukin-1β(IL-1β), IL-6, IL-8. tumor necrosis factor(TNF) and NO in the supernatants of keratinocyte culture in which culture media containing 25, 50, 100, 200, 400 μg/ml, respectively of aloe polysaccharide were assayed. In the control group equal volume of media without the polysaccharide was used. Results Compared with control group, the levels of TGF-α, TGF -β1, IL - 1β, IL-6, IL-8 and TNF in the supernatants of cultured keratinocytes were significantly higher when aloe polysaccharide was added (P<0. 05 or P<0. 01), and they were positively correlated to the concentration of aloe polysaccharide (P<0. 01). However, aloe polysaccharide markedly decreased the level of NO in a dose dependent manner (P<0. 01). Conclusion Aloe polysaccharide could promote keratinocytes to secrete TGF-α, TGF-β1, IL-1β, IL - 6, IL - 8 and TNF, and inhibit the release of NO.