普伐他汀抑制重组人CD40配体诱导的U937诱导型细胞环氧合酶的表达

Pravastatin inhibits cyclooxygenase-2 expression induced by rhCD40L in cultured U937 cells

目的 探讨重组人CD40 配体(rhCD40L)对培养的人单核细胞(U937)表达诱导型环氧合酶(COX 2)的作用和普伐他汀对其的影响。方法 体外培养U937 细胞,分别以浓度为0. 05、0. 10、0. 20、0.40μg/ml的rhCD40L刺激24 h;以0. 40μg/ml的rhCD40L 分别刺激3、6、12、24 h。再将0. 40μg/ml 的rhCD40L与终浓度分别为1×10-2、1×10-3、1×10-4 mmol/L的普伐他汀共同刺激24 h。采用逆转录聚合酶链反应(RT PCR)检测COX 2 mRNA的表达。结果 0.4μg/ml的rhCD40L刺激后3 h,可诱导COX 2 mRNA表达,随着rhCD40L浓度和作用时间增加,COX 2 mRNA表达明显增加(P<0.01),呈时效和量效的关系。0.4μg/ml的rhCD40L与普伐他汀共同刺激24 h后,COX 2 的表达明显被抑制。结论 rhCD40L可以时间和浓度依赖的方式诱导COX 2的表达,普伐他汀可抑制此作用。

Objective To evaluate the cyclooxygenase-2(COX-2) expression induced by rhCD40L in cultured U937 cell and the possible inhibition by pravastatin. Methods The U937 cells were cultured, co-incubated with rhCD40L in certain dosage and duration with or without pravastatin. The COX-2 expressions induced by rhCD40L were detected by RT-PCR. Results rhCD40L increased the expression of COX-2 mRNA in a time- and dose-dependent manner (P< 0.01). Pravastatin was founed to inhibit the expression of COX-2 mRNA induced by rhCD40L in a dose-dependent manner (P< 0.01). Conclusion rhCD40L can induce cyclooxygenase-2 expression in U937 cells with dose and time-dependent manners, and pravastatin can inhibit this enhanced expression.