亚砷酸和STI571单用及联用对K562细胞增殖和凋亡的影响

Effects of STI571 Alone and in Combination with As_2O_3 on Proliferation and Apoptosis of the K562 Cells

目的 研究亚砷酸(As2 O3 )、STI5 71单用及联用对K5 6 2细胞增殖和凋亡的影响,为As2 O3 和STI5 71联合应用于临床提供理论依据。方法 细胞增殖采用细胞生长及活力测定;细胞凋亡采用细胞形态、Annexin V/PI双染实验、DNA的PI染色及DNA电泳等方法测定。结果 在As2 O3 和(或)STI5 71作用下K5 6 2细胞生长受抑伴随活力下降,流式细胞仪检测出凋亡细胞百分率呈时间剂量依赖关系,基因组DNA电泳出现“梯”状条带。结论 As2 O3 和STI5 71均有抑制K5 6 2细胞增殖和诱导该细胞凋亡的作用,两药联用效果更佳。

Objective To investigate the effects of STI571 alone and in combination with As_2O_3 on proliferation and apoptosis of the K562 cells and provide theoretical basis for clinical application. Methods Cell proliferation was analyzed by the growth of cell and the viability of cell; Apoptosis was analyzed by cell morphology, Annexin-V/PI staining, DNA-PI staining, and DNA gel electrophoresis. Results After exposure to As_2O_3 and/or STI571, the growth of K562 cells was inhibited and the viability of K562 cells was decreased. The percentage of apoptotic cells was in time-and dose-dependent manner. Typical DNA ladder was shown by DNA gel electrophoresis. Conclusion As_2O_3 and STI571 inhibit the proliferation of K562 cells and induce apoptosis of the K562 cells, the combination of the two drugs has better results.