摘要
目的 探讨金克(槐耳清膏)在胰腺癌细胞Panc -1生长转移过程中的影响及可能机制。方法 不同浓度的金克及5 - FU分别与人胰腺癌细胞Panc -1共同培养。MTT法观察生长抑制曲线,FCM观察细胞周期变化及凋亡发生,TUNEL法观察凋亡发生情况并计算凋亡率,细胞免疫组化检测凋亡基因Caspase -3的表达,ELISA法检测细胞培养上清VEGF的分泌变化。结果 金克抑制Panc -1的生长,主要阻滞在G1期,促进凋亡发生、Caspase -3的表达并抑制VEGF的分泌。结论 金克对于胰腺癌细胞Panc -1有确切杀伤抑制疗效。
Objective To investigate the influence of growth and metastasis induced by Jinke in human pancreatic adenocacinoma and possible mechanism. Methods Jinke and 5-FU were cultured seperatively with human pancreatic adenocacinoma cell line Panc-1. Cell growth was assessed by MTT. FCM and TUNEL showed the change of cell cycle and apoptosis rate.Cell immunohistochemistry showed the change of Caspae-3 expresion.While VEGF secretion was assessed by ELISA. Results Jinke inhibited the growth of Panc-1 cell,mainly in G_1 stage,induced apoptosis and Caspase-3 expression while inhibted VEGF secretion. Conclusion Jinke is useful in the therapy of pancreatic adenocarcinoma cell Panc-1.
出处
《苏州大学学报（医学版）》
CAS
北大核心
2005年第2期226-228,240,共4页
Suzhou University Journal of Medical Science
