低密度脂蛋白免疫复合物对单核细胞衍生巨噬细胞中丙二醛产生的影响

Effects of LDL-containing immune complexes on production of MDA in monocyte-derived macrophages

目的:探讨低密度脂蛋白免疫复合物(L DL- IC)对单核细胞衍生巨噬细胞中丙二醛(MDA)产生的影响。方法:采用比色法测定培养上清MDA含量。观察不同浓度L DL - IC和不同作用时间巨噬细胞MDA产生的变化,并设立终浓度均为15 0 μg/ ml的L DL- IC、L DL、Ig G- IC及阴性对照组与细胞孵育36 h,比较各组培养上清MDA水平的差异。结果:MDA水平随着L DL - IC的作用浓度与孵育时间增加而升高。与阴性组相比,L DL - IC、L DL及Ig G- IC均能明显提高上清MDA含量,而L DL- IC的作用最明显(P<0 .0 1)。结论:L DL- IC可以显著增加巨噬细胞MDA的产生,提示L DL - IC能增加活性氧物质而在动脉粥样硬化中起重要作用。

Objective:To study the effect of LDL-containing immune complexes(LDL-IC) on the malondialdehyde production in monocyte derived macrophages.Methods:To investigate whether there was any dose or time dependent effect on the MDA production,PMA-treated THP-1 cells were incubated with different concentrations of LDL-IC(0、50、100、150、200、250μg/ml)for 36h or treated with 150μg/ml LDL-IC for different hours(0、3、6、12、24、36h)。Moreover,the luminescence level of MDA of the four matched groups of LDL-IC,native LDL,immune complexes of IgG(IgG-IC) and negative control was measured respectively.Results:The MDA levels were correlated with both LDL-IC concentration and incubating time.The MDA levels of the macrophages incubated with LDL-IC,native LDL,IgG-IG were all elevated markedly compared to negative control(P<0.01),but the increased level of the group incubated with LDL-IC was the highest(P<0.01).Conclusions:LDL-IC have the greatest capacity to promote the production of MDA.LDL-IC may play an important role in the development of atherosclerosis by reactive oxygen species release.