基因芯片检测血清标本的丙型肝炎病毒基因型

The genotypes of hepatitis C virus detected by HCV DNA microarray

目的:探讨丙型肝炎病毒(HCV )基因分型检测芯片在临床的初步应用及意义。方法:基因芯片检测15 0例血清标本中HCV基因型,并与HCV RNA定性及测序结果进行比较。结果:HCV基因分型检测芯片检出的阳性结果与HCV- RNA定性及测序结果的相对符合率为10 0 .0 %。结论:HCV基因分型检测芯片是一种准确性好、灵敏度高、特异性强、操作简便快速的基因检测方法,可以为临床诊断。

Objective:To approach the primary application and significance of HCV genotyping chip in the clinical practice.Methods:The results from 150 serum samples detected by HCV genoyping chip were compared with those from traditional RT-PCR and sequencing.Results:There was 100.0% coincidence between HCV genotyping chip and the others.Conclusions:HCV genotyping chip is a fast and easy method for genotyping which shows higher specificity and sensitivity,so it can play an important part in the diagnosis and therapy.