固定化丙酸菌的初步研究

Study on the Immobilization of Propionibacteria

对丙酸菌固定化的条件进行了研究,固定于海藻酸钠、琼脂、卡拉胶3种载体上的丙酸菌的分批发酵的结果表明,海藻酸钠包埋丙酸菌活性最高。在文中实验条件下,用于固定化的最适海藻酸钠的浓度为4 %。固定化颗粒的直径大小,固定化细胞的质量与发酵液体积比等对产酸速率都有影响。4℃储存4 5d的固定化细胞,其发酵产丙酸活性经4批实验后仍与储存前基本相同。在最适条件下,连续13批次的发酵实验表明,固定化细胞稳定,凝胶颗粒强度无变化,适于连续化生产。与游离丙酸菌比较,固定化细胞的丙酸生成速度加快。

In this paper the immobilized propionibactera were investigated Three carriers were tested for immobilized propionibacteria, the batch experiment results shown that the sodium alginate was the best one of the three immobilized material In the laboratory-scale experiments, we observed that the optimum concentration of the sodium alginate was 4% We examed the effect of the size of the immobilized beads, the rate of the weight of the immobilized beads /the volume of the medium on the productive acid rate After the immobilized cells were maintainted for 45 days at 4℃, their vigorous ability of the immobilized cells after 4 batches was the same as the before Under the optimum conditions,14 batches of fermentation performed contiuously showed that, the bioactivities of the immobilized cells and the mechanical strength of the gel grains were constant, therefore it could be used in a continuous production The observed productive acid rate and its yield of immobilized cells appear to be greater than the corresponding cells in suspension