叶黄素的体外抗氧化功能

Study on the Singlet-oxygen Quenching of Lutein from Marigold Flower in vitro

应用反相高压液相色谱(RP HPLC)从万寿菊的花中制备叶黄素的反式(E )、顺式(Z )异构体及其酯类3个组分。然后,应用微弱发光检测技术测定和比较各组分在体外淬灭单线态氧的能力。结果表明,反式和顺式叶黄素及其酯类均有体外淬灭单线态氧的能力,同时,还可观察到顺式异构体的“延迟发光”现象。

Three fractions, all E- and Z-isomers of lutein and their esters were prepared by reversed phase HPLC from the flower of marigold Examination and comparison of the singlet-oxygen quenching ability by those fractions were undertaken and analyzed by ultra weak luminescence It was observed that all of three fractions, esters, all E- and Z-isomers, could extinguish singlet-oxygen in vitro Additionally, ‘Time delay' effect of Z-isomers was observed This observation suggested that Z-isomers quenched singlet-oxygen with a different mechanism from all E-isomer Application of those fractions in functional food and pharmaceutical products with anti-oxidation function might be considered based on those observations