过氧化物酶体增殖剂激活受体α对大鼠胆汁酸合成代谢的影响

EFFECT OF PEROXISOME PROLIFERATOR-ACTIVATED RECEPTOR-α ON BILE ACID BIOSYNTHESIS IN RATS

目的探讨过氧化物酶体增殖剂激活受体α(peroxisomeproliferator activatedreceptorα,PPARα)对大鼠胆汁酸合成的影响。方法采用邻苯二甲酸二异辛酯(diethylhexylphthalate,DEHP)激活PPARα,测定血清三酰甘油、总胆固醇及粪胆汁酸的水平;用逆转录酶聚合酶链反应(reversetranscriptase polymerasechain reaction,RT PCR)法测定肝脏PPARα、胆固醇7α羟化酶(cholesterol7αhydroxylase,CYP7A1)mRNA水平。结果应用DEHP后血清三酰甘油降低,肝脏PPARαmRNA表达增加;同时肝脏CYP7A1mRNA表达加强,粪胆汁酸排出显著增加。结论PPARα的激活增加CYP7A1mRNA表达及粪胆汁酸排出,对胆汁酸的合成具有调节作用。

ObjectiveTo determine the physiological role of peroxisome proliferator-activated receptor-α(PPAR-α) in the bile acid biosynthesis. MethodsPPAR-α was induced in rats by diethylhexyl phthalate(DEHP). The levels of serum triglyceride, total cholesterol and fecal bile acids were measured.The mRNA contents of hepatic PPAR-α and cholesterol 7α-hydroxylase(CYP7A1) were detected by reverse transcriptase-polymerase chain reaction(RT-PCR). ResultsIn DEHP fed rats,the mRNA content of PPAR-α was increased significantly together with the serum triglyceride decreased,and at the same time the mRNA content of CYP7A1 and the fecal bile acids were increased strongly. ConclusionPPAR-α increased CYP7A1 mRNA expression and the fecal bile acids showing that PPAR-α plays a role in the regulation of bile acid biosynthesis.