HCV感染者和HCV/HIV合并感染者免疫调节

Analysis on CD4^+CD25^+Tcell in HCV infection and HCV/HIV co-infection

目的 探讨我国单纯丙型肝炎病毒(HCV)感染者和HCV/HIV合并感染者免疫应答的相关机制。方法 分离人外周血单个核细胞;流式细胞仪(FACS)检测CD4 + T细胞和CD4 + CD2 5 + T细胞表达水平。结果 CD4 +CD2 5 + T细胞占外周血单个核细胞中CD4 + T细胞比例(%CD4 + CD2 5 + ) ,HCV感染组明显高于健康对照组(19. 2 %>13 8% ,P <0 . 0 5 ) ,HCV/HIV合并感染组明显低于对照组(6. 9% <13. 8% ,P <0 .0 0 1) ,更明显低于HCV感染组(P <0 . 0 0 1)。结论 HCV感染者体内CD4 + CD2 5 + T细胞增殖明显,提示CD4 + CD2 5 + T细胞在HCV慢性感染中发挥免疫调节作用。HCV/HIV合并感染时,CD4 + CD2 5 + T细胞受损,从而影响免疫调节功能。

Objective To investigate the characteristic of cellular i mmune responses in chronic HCV patients and HCV/HIV coinfection patients from Ch ina.Methods Human peripheral blood monocytes were isolated.Th e frequency and percentage of CD4+T lymphocytes and CD4+CD25+T lymphocytes were determined by FACS.Results The higher proportion of CD4+CD25+T lymphocyt es was found in chronic HCV infection than that of normal control(19.2%>13.8%, P<0 .05),while significant lower percentage was found in HCV/HIV coinfection than n ormal control(6.9%<13.8%,P<0.001)or chronic HCV infection(P<0.001).Conclusion The individuals have a strong CD4+CD25+T c ells proliferation against HCV infection while a week CD4+CD25+T cells again st HCV/HIV co-infection.It suggests that CD4+CD25+T lymphocytes plays an im mune regulatory role in HCV persistence.HCV/HIV co-infection would impair the C D4+CD25+T lymphocytes,the immune regulatory function would be affected.