共轭亚油酸对肥胖大鼠UCP2基因表达的影响

Effect of conjugated linoleic acid on gene expression of UCP2 in obese rat fed with high fat diet

目的 通过研究共轭亚油酸(Conjugatedlinoleicacid ,CLA)对饮食诱导肥胖大鼠解偶联蛋白2 (Uncou plingprotein 2 ,UCP2 )基因表达的影响,探讨CLA降低体重、体脂作用的机制。方法 选用雄性Wistar大鼠,随机分为对照组、高脂组、高脂+CLA组(每10 0g饲料含CLA分别为0 . 75 ,1 .5 0 ,3 .0 0g) ,每组动物10只,观察CLA对肥胖大鼠体重、体脂、血脂水平的影响,并应用RT PCR的方法检测UCP2mRNA的表达水平。结果 CLA可降低饮食诱导肥胖大鼠的体重和体脂含量,降低血清甘油三酯、胆固醇、游离脂肪酸水平,并可增加肥胖大鼠脂肪组织UCP2mR NA的表达水平。结论 CLA可改善肥胖大鼠的脂代谢紊乱,增加UCP2基因的表达,发挥降低体重、体脂的作用。

Objective To study the effect of conjugated linoleic acid (CLA)on expression of Uncoupling protein 2(UCP2)in white adipose tissue of obese rats,and to explore the mechanism of reduce the body weight and fat content of obeses rats by CLA.Methods Male Wistar rats were randomly separated to contr ol group,high-fat group and high fat+CLA group(~0.75?g,~1.50?g,~3 .00?g% by deit weight).The effect of conjugated linoleic aci d on body weight,fat content and blood lipids were observed.Using reverse transcript ion polymerse chain reaction(RT-PCR)technique to measure the mRNA expression level of UCP2.Results CLA reduced the body weight,fat content,decreased serum triglyceride,cholesterol,free fatty acid levels,and increased the express ion of UCP2 mRNA in adipose tissue of obese rat.Conclusion CLA can improve dyslipidaemia of obese rat and reduce weight body and fat content by increasing the expression of UCP2 mRNA.