乙肝病毒DNA水平与表面抗原滴度水平比较

Relativity between HBV DNA and HBsAg titer.

目的 比较荧光定量PCR检测乙肝病毒与表面抗原滴度检测的结果符合率,探讨PCR检测HBVDNA方法的应用前景。方法 抽取服务行业从业人员体检血清10 2 5份,进行HBV荧光定量PCR和ELISA结果以及表面抗原滴度检测结果的比对分析,以确认标准方法。结果 荧光PCR与乙肝两对半ELISA结果相比符合一般规律,而PCR检测结果与表面抗原滴度结果无明显相关性。结论 HBVDNA定量对治疗及确认是否具传染性起指导作用,可以取代表面抗原滴度检测方法对E抗原阴性而表面抗原阳性的服务行业从业人员进行传染性判定。

Objective To compare the correlation of results of HBV DN A by florescence PCR and HBsAg titer,and to discuss the application of detecting HBV DNA by fluorescent quantitative PCR.Methods The serum samples from ~1?025 HBsAg positive persons applying for service work were tested by FQ-PCR kits and compared with ELISA results and HBsAg titer test.Results The results of FQ-PCR comparing with ELISA were accord with the general rule.The results of FQ-PCR comparing with HBsAg titer t est had no significantly relationship.Conclusion The quantification of HBV DNA can be a referen ce for anti-virus therapy and evaluation of infectivity.Instead of the HBsAg ti ter test,workers of food production and sell with HBsAg positive would be better tested on HBV DNA for the application of health certification.