大鼠肠内菌对麦冬皂苷D′代谢的研究

Effect of intestinal bacteria on metabolism of ophiopognin saponin D′ in rats

目的:通过大鼠肠内菌代谢转化麦冬皂苷D′的离体、在体实验,探索麦冬皂苷D'口服后吸收入血成分。方法:采用HPLC ELSD定量检测离体实验的代谢产物及原形药含量;采用TLC ,HPLC -MS法分别鉴定、监测在体实验大鼠尿、血中的代谢产物。结果:麦冬皂苷D′可被大鼠肠内菌群代谢,且随着时间的延长,代谢产物之一薯蓣皂苷元含量增加。结论:薯蓣皂苷元为麦冬皂苷D′经肠内菌代谢后的吸收入血成分之一。

Objective: To explore the metabolic transformation and the absorbed metabolites of ophiopognin saponin D′(OD′) (given orally) in rats. Method: The contents of both original OD′ and its metabolites were detected by means of HPLC-ELSD and the metabolites of OD′ in blood and urine were measured by use of TLC and HPLC-MS in vivo. Result: OD′ could be metabolized by intestinal bacteria in rats. The content of diosgenin, one of the metabolites,increased gradually as the time passed. Conclusion: OD′ can be metabolized in intestine of rat and its metabolite, diosgenin, was absorbed in blood of rat.