大孔吸附树脂分离富集土贝母总皂苷的研究

Isolation and purification of total tubeimosides from Bolbostemma paniculatum by macroporous resin

目的:优化大孔吸附树脂分离纯化土贝母总皂苷的工艺条件。方法:以土贝母总皂苷的吸附量和解吸率为指标,大范围筛选树脂,并通过单因素试验、正交试验及方差分析确定了土贝母总苷分离富集的优选操作条件。结果:NKA树脂具有较好的吸附性能和解吸效果;优选吸附条件为上样含生药量30mg·mL-1,树脂色谱柱的径高比1∶7,药材∶树脂为1∶4;吸附完全后,先以水洗脱,除去杂质,再以80%乙醇洗脱,可以得到纯度较好的土贝母总苷。结论:NKA树脂对土贝母总苷的吸附量大,解吸容易,确定的吸附与洗脱条件简单可行,所得产品的色泽好、纯度高,此工艺具有良好的产业化前景。

Objective: To select the optimum purification process of all tubeimoisides from Bolbostemma paniculatum by macroporous resin. Static absorption test and deabsorption test were carried out to screen the best macroporous resin for all tubeimosides. The single factor test, orthogonal experimental designs and variance analysis were applied to optimize the manipulation parameters of macroporous resin. Result: The macroporous resin NKA possessed the strongest absorption ability to all tubeimosides among the 9 resins studied, in addition to an easy de-absorption property. The optimum absorption conditons were A_(2)B_(3)C_(1), namely concentration of feede 30 mg·mL^(-1), diameter: height of the chromatography column 1∶7. the weight ratio of raw material and resins 1∶2. Conclusion: The above experimental results can provide experimental basis for large-scale purification process of all tubeimosides.