SPR生物传感器在生命科学及在航天医学中的应用

Applications of Surface Plasmon Resonance Biosensor in Life Science and Space Medicine

表面等离子体共振生物传感器在分析生物分子相互作用方面,具有灵敏度高、实时在线、简单快捷和抗干扰能力强等优点,被广泛应用于临床检验、药物筛选等研究领域;因其能满足空间特殊环境下的使用要求,在航天医学领域具有广阔的应用前景.本文综述了表面等离子体共振生物传感器的原理、特点、在生命科学研究中的应用及在航天医学中的应用前景.

The use of Surface Plasmon Resonance (SPR) biosensors in analyzing biomolecular interactions is increasing significantly in recent years, since it has various advantages including real-time measurement of biomolecular interactions without labeling, high-throughput analysis of multiple proteins and the simple optical system for the device. Applications in life science include clinical assay, disease diagnosis, drug screening and so on. In this technique, one of the interacting partners is immobilized on a sensor chip and the binding of the other is followed by the increase in refractive index caused by the mass of bound species. Developments of related theories and technologies have accelerated performance of SPR biosensor greatly. For its high sensitivity, facility, expeditiousness and stable ability in weightlessness, SPR biosensor has a strong potential for on-line analyzing biochemical markers in astronauts' body fluids which are highly related to some kinds of diseases, and this is a practical method used as the health early alarm and medical care system for astronauts. This article is focused on the principle and advantages of SPR-based biosensor and its applications in life science. The future uses in space medicine are also discussed.