摘要
以兔胸腺可提取性核抗原(ENA)的免疫印迹技术(IBT),对177例结缔组织病患者及32例正常人血清进行了7种自身抗体的测定。抗Sm抗体见于50%的SLE患者,敏感性较高;抗RNP抗体可在多种结缔组织病中测出,在SLE中阳性率较高;抗Ro(SSA)、抗La(SSB)抗体见于部分SLE患者;抗核糖体抗体仅见于SLE,可能为SLE的又一标记。本文IBT具有广谱、快速、精确及稳固等优点,对提高结缔组织病的诊断和鉴别诊断水平有重要意义。
Immunoblotting technique (IBT ) against extractable nuclear antigen (ENA) from rabbit thymuswere applied to detect seven autoantibodies in sera from177 patients with connective tissue diseases and 32healthy blood doners. Anti-Sm antibody was found in50% of 145 patients with SLE. Anti-RNP antibody existed in sera of several connective tissue diseases andshowed higher sensitivity in SLE (58% ). 16 % and 5 %patients with SLE Were anti-Ro (SSA ) and anti-La(SSB) positive respectively. The most striking findingwas that anti-ribosomal antibody was positive (13%)only in SLE and should provide a new marker for diagnosis of this disease. These data suggest that IBT ishelpful not only in raising the sensitivity of SLE, butalso in distinguishing connective tissue diseases fromeach other.
出处
《中国皮肤性病学杂志》
CAS
北大核心
1994年第4期207-209,共3页
The Chinese Journal of Dermatovenereology
关键词
免疫印迹技术
抗体
红斑狼疮
系统性
胶原病
Immunoblotting technique Antibody Connective tissue disase Lupus erythematosus, systemic