免疫印迹技术检测七种结缔组织病自身抗体

Seven Autoantibodies Detected by Immunoblotting Technique in Connective Tissue diseases

以兔胸腺可提取性核抗原（ＥＮＡ）的免疫印迹技术（ＩＢＴ），对１７７例结缔组织病患者及３２例正常人血清进行了７种自身抗体的测定。抗Ｓｍ抗体见于５０％的ＳＬＥ患者，敏感性较高；抗ＲＮＰ抗体可在多种结缔组织病中测出，在ＳＬＥ中阳性率较高；抗Ｒｏ（ＳＳＡ）、抗Ｌａ（ＳＳＢ）抗体见于部分ＳＬＥ患者；抗核糖体抗体仅见于ＳＬＥ，可能为ＳＬＥ的又一标记。本文ＩＢＴ具有广谱、快速、精确及稳固等优点，对提高结缔组织病的诊断和鉴别诊断水平有重要意义。

Immunoblotting technique (IBT ) against extractable nuclear antigen (ENA) from rabbit thymuswere applied to detect seven autoantibodies in sera from177 patients with connective tissue diseases and 32healthy blood doners. Anti-Sm antibody was found in50% of 145 patients with SLE. Anti-RNP antibody existed in sera of several connective tissue diseases andshowed higher sensitivity in SLE (58% ). 16 % and 5 %patients with SLE Were anti-Ro (SSA ) and anti-La(SSB) positive respectively. The most striking findingwas that anti-ribosomal antibody was positive (13%)only in SLE and should provide a new marker for diagnosis of this disease. These data suggest that IBT ishelpful not only in raising the sensitivity of SLE, butalso in distinguishing connective tissue diseases fromeach other.