摘要
为探讨Inhib-ELISA法推广应用的可能性,本文采用该法与PHA法连续3年对我省各鼠疫疫区送检复判的222份血清标本进行了平行检测。结果显示,Inhib-ELISA检出阳性血清152份,阳性检出率为68.5%,几何平均滴度为1:434.51;而PHA法共检出阳性血清128份,阳性检出率为57.7%,几何平均滴度为1:68.60,两者相差均具有非常显著性意义。且PHA初判时出现4.5%的假阳性反应,而Inhib-ELISA无一例假阳性反应。结果表明,Inhib-ELISA比PHA法具有结果稳定、检出率高、敏感性好和特异性强等特点。连续三年的应用实践说明,该法若能在我国鼠疫防治工作中推广应用,必将会提高我国鼠疫监测工作的质量。
The inhibit enzyme linked immunosorbent assay(ELISA) and passive hemagglutination assay (PHA)have been used in the testing of serum samples from plague endemic areas in Qinghai continuously for three years,and the possibility of popularizing of the Inhib-ELISA was inquired,222 samples from the endemic areas were parallelly tested by using the two assays. The results showed that 152 Samples were positive by the Inhib-ELISA,with the positive rate of 68.5% and the average geometric titre of 1: 434.51, without the false positive result.As comparison,128 Samples were positive by the PHA. with the positive rate of 57.7%,the false rate of 4.5%,and the average geometric titre of 1: 68.6. The significant differences existed between the results of the two methods,It was suggested that the Inhib-ELISA should be more stable,sensitive,specific and more truth positive rate than PHA and It proved by three years practices that the quality of plague surveillance would be improved if the Inhib-ELISA method can be popularized in the plague control work in China.
出处
《中国人兽共患病杂志》
CSCD
北大核心
1994年第4期16-18,共3页
Chinese Journal of Zoonoses