ELISA抑制法及被动血凝试验对鼠疫血清标本检测结果的比较分析

Comparison on Testing Results of Plague Serum Samples Between Inhib-ELISA and PHA

为探讨Ｉｎｈｉｂ－ＥＬＩＳＡ法推广应用的可能性，本文采用该法与ＰＨＡ法连续３年对我省各鼠疫疫区送检复判的２２２份血清标本进行了平行检测。结果显示，Ｉｎｈｉｂ－ＥＬＩＳＡ检出阳性血清１５２份，阳性检出率为６８．５％，几何平均滴度为１：４３４．５１；而ＰＨＡ法共检出阳性血清１２８份，阳性检出率为５７．７％，几何平均滴度为１：６８．６０，两者相差均具有非常显著性意义。且ＰＨＡ初判时出现４．５％的假阳性反应，而Ｉｎｈｉｂ－ＥＬＩＳＡ无一例假阳性反应。结果表明，Ｉｎｈｉｂ－ＥＬＩＳＡ比ＰＨＡ法具有结果稳定、检出率高、敏感性好和特异性强等特点。连续三年的应用实践说明，该法若能在我国鼠疫防治工作中推广应用，必将会提高我国鼠疫监测工作的质量。

The inhibit enzyme linked immunosorbent assay(ELISA) and passive hemagglutination assay (PHA)have been used in the testing of serum samples from plague endemic areas in Qinghai continuously for three years,and the possibility of popularizing of the Inhib-ELISA was inquired,222 samples from the endemic areas were parallelly tested by using the two assays. The results showed that 152 Samples were positive by the Inhib-ELISA,with the positive rate of 68.5% and the average geometric titre of 1: 434.51, without the false positive result.As comparison,128 Samples were positive by the PHA. with the positive rate of 57.7%,the false rate of 4.5%,and the average geometric titre of 1: 68.6. The significant differences existed between the results of the two methods,It was suggested that the Inhib-ELISA should be more stable,sensitive,specific and more truth positive rate than PHA and It proved by three years practices that the quality of plague surveillance would be improved if the Inhib-ELISA method can be popularized in the plague control work in China.