摘要
探讨复方鳖甲软肝片对5 /6肾切除(5 /6NT)大鼠肾组织内皮素(ET- 1)mRNA表达的影响。120只SD大鼠随机均分为正常组,假手术组, 5 /6NT模型组,苯那普利组,尿毒清组和复方弊甲软肝片大、中、小剂量组。连续用药28周后,处死大鼠,无菌取肾,制作肾组织冰冻制片,用原位杂交方法检测各组ET 1mRNA的表达。结果:正常组、假手术组,仅见少量ET- 1mRNA阳性反应物; 5 /6NT模型组,肾小球系膜细胞浆、肾小管上皮细胞浆均见ET -1mRNA强阳性蓝色反应物;苯那普利组、尿毒清组ET -1mRNA阳性反应物减少,与5 /6NT组比,P<0. 05;复方鳖甲软肝片ET -1mRNA阳性反应物明显减少,并呈剂量依赖关系;与5 /6NT组比,P<0. 05。结论:复方鳖甲软肝片能抑制5 /6NT大鼠肾组织ET- 1mRNA的表达,这可能是该方防治肾小球硬化的作用机制之一。
To study the effect of 'Fufang Biejia Ruangan Tablet' on E T-1 mRNA expression in kidney tissue in rats of five-sixths nephretomy (5/6NT) , SD rats were randomized into normal group, sham group, model group, bennapuli group, niaoduqing group, Tablet low-dose group, Tablet medium-dose group and T ablet high-dose group. After 28-week consecutive corresponding treatment, the rats were sacrificed and kidney tissues were taken away to assay the ET-1 mRNA expressions. Results: In normal group and sham group, there were a few ET-1 mRNA positive reactants; in model group, there were more ET-1 mRNA pos itive reactants; in bennapuli group and niaoduqing group, ET-1 mRNA positive re actants reduced, with a difference in comparison with model group (P<0.05); in the three Tablet groups, the ET-1 mRNA positive reactants reduced obviously, with a difference with model group(P<0.05), depending on the dose. Conclusi on: The 'Fufang Biejia Ruangan Tablet' may inhibit the ET-1 mRNA expression t o prevent and treat glomerular sclerosis.
出处
《上海中医药杂志》
北大核心
2005年第5期49-51,共3页
Shanghai Journal of Traditional Chinese Medicine
基金
国家自然科学基金重点资助项目 ( 30130220 )
教育部"长江学者和创新团队发展计划"资助项目(IRT0413)
国家中医管理局中医药科研基金资助项目(2000J P 31)。
