RP-HPLC法测定蒙药那如-3丸中乌头类生物碱含量

Determination of alkaloids in the Mongolian recipe Naru-3 pill by RP-HPLC

目的建立测定蒙药那如 3丸中乌头类生物碱含量的RP HPLC法。方法采用C18色谱柱,以甲醇 水 氯仿 三乙胺(体积比6 0∶4 0∶2∶0 1)为流动相,流速0 8mL·min-1,柱温为室温,检测波长2 5 4nm ,进样体积10 μL,外标法计算含量。结果乌头碱、中乌头碱和次乌头碱在0 3～1 5g·L-1内峰面积与质量浓度呈良好线性关系,相关系数r分别为0 9996、0 9998、0 9998,平均回收率分别为10 1 0 %、10 0 9%、10 0 3% ,RSD分别为1 9%、1 2 %、1 4 % (n =9) ;测得那如 3丸中乌头碱、中乌头碱和次乌头碱的质量分数分别为0 0 0 12 6 %、0 0 0 2 78%、0 0 0 335 %。结论RP HPLC法可用于那如 3丸中乌头类生物碱的质量控制。

Objective To establish a RP-HPLC method for the determination of the content of alkaloids in the Mongolian recipe Naru-3 pill.Method The content of alkaloids in Naru-3 pill was determined by RP-HPLC with Hypersil C 18 column at room temperature. The mobile phase was methanol-water-chloroform-triethylamine (V∶V∶V∶V∶=60∶40∶2∶0.1) with a flow rate of 0.8 mL·min~ -1 ,the injection volume was 10 μL .The external standard was used and the wavelength of the detector was set at 254 nm .Results The linear range was 0.3～ 1.5 g·L~ -1 ,the correlation coefficients were 0.999 6 , 0.999 8 ,and 0.999 8 .The average recoveries and RSD were 101.0%,100.9%,100.3%,and 1.9%,1.2%,1.4%,respectively.Conclusion The method was proved to be convenient and accurate and can be used for the quality control of the Mongolian recipe Naru-3 pill.