摘要
目的观察联合使用环氧化酶抑制剂阿斯匹林(Asp)和β-内啡肽受体拮抗剂纳络酮(Nal)对腹腔感染大鼠全胃肠外营养(TPN)时蛋白质分解的调理作用。方法将28只成年腹腔感染大鼠随机分成对照组(TPN组,n=9)和实验组(Asp+Nal组,n=12),对照组给予常规TPN,实验组在TPN溶液中加入注射用Asp15mg/(kg·d)和Nal注射液1mg/(kg·d),持续4d。测定实验前后体重、血清总蛋白、白蛋白、尿素氮和肌酐的浓度,测定每日氮平衡和尿3-甲基组氨酸(3-MH)的排出量,计算累积氮平衡和累积尿3-MH排出量。结果实验组血清学指标实验前后变化的差值比对照组显著减少,累积氮平衡显著优于对照组(P<0.01),累积尿3-MH排出量比对照组显著减少(P<0.01)。结论联合使用环氧化酶抑制剂Asp和β-内啡肽受体拮抗剂Nal可以调理腹腔感染大鼠TPN时的蛋白质代谢,减少蛋白质丢失。
Objective To investigate the effects of cyclo-oxygenase inhibitor aspirin(Asp)combined with β-en-dophine receptor inhibitor Naloxone(Nal)on protein metabolism in total parenteral nutrition(TPN)in rats. Methods Twenty-eight SD rats with abdominal infection were randomly divided into control group(TPN group,n = 9)and experimental group(Asp+Nal+TPN,n = 12). The total nutritional admixture(TNA)was infused to every rat in each of the group. Asp 15 mg/(kg·d)and Nal 1 mg/(kg·d)were given in TNA in experimental group. The study lasted for 4 days. Body weight,total serum protein,serum albumin,blood urinary nitrogen(BUN),and creatine(Cr)were determined at the beginning and the end of the study. Nitrogen balance(NB)was estimated daily and the amount of urinary 3-MH were calculated at the end of this study. Results The variety of serological index significantly decreased in experimental group than that in control group. Accumulated NB was increased and accumulated 3-MH decreased significantly in the experimental group. Conclusion Asp combined with Nal may intervene protein metabolism in TPN and reduce protein loss in sepsis.
出处
《中国临床营养杂志》
2005年第2期96-99,共4页
Chinese Journal of Clinical Nutrition
