部分耐盐小麦品种(系)SSR位点遗传多样性研究

Genetic Diversity of Some Salt-tolerant Wheat ( Triticum aestivum L.) Cultivars Detected by SSR Markers

选择有多态性的32对SSR引物对80个小麦耐盐品种(系)进行遗传差异研究,共检测出155个等位变异,平均每个位点上有4.75个等位变异;供试80份耐盐小麦品种(系)来源广泛,遗传基础丰富,表现出较高的遗传多样性,遗传相似系数范围在0.26-0.81;聚类分析结果显示,冬性小麦品种(系)聚为一大类;春性小麦品种(系)也聚为一大类;一些系谱相同或相近的品种(系)遗传相似系数较大;A、B、D基因组中SSR位点平均等位变异差异不大,以B基因组较高。

Genetic diversity is important for breeding programs. Thirty-two selected microsatellite markers were used to detect genetic diversity among 80 salt-tolerant wheat cultivars. Totally 155 alleles were revealed among the 80 accessions. The number of alleles per SSR loci varied from 1 to 12, most of them from 4 to 8 and with the average of 4.75. The genetic distance among the 80 accessions varied from 0.26 to 0.81. Cluster analysis among the 80 accessions with salt tolerance showed that all the cultivars were clustered into two groups, that is the winter wheat group and the spring wheat group. Most accessions with same pedigree backgrounds were clustered into one subgroup. The difference of allelic variation in genome A, B and D was small, but genome B had the highest number of alleles.