几个中国大麦属物种叶绿体psbL和psbJ基因序列分析

Sequence Analysis of Chloroplast psbL and psbJ Genes of Some Hordeum Species from China

对8份来源于中国和2份来自国外的不同大麦属物种或亚种叶绿体基因组psbL-psbJ区进行了序列测定。结果表明,10份大麦属材料的psbL-psbJ区序列长度均为365bp,且无变异位点。同时,将大麦属psbL-psbJ区序列与已报道的黑麦(Secalecereale L.)、小麦(Triticumaestivum L.)、水稻(Oryza sativa L.)和玉米(Zea mays L.)的相同序列比较,发现所有物种的psbL基因序列长度均为117bp,且无核酸变异位点;psbJ基因序列长度均为123bp,共检测到6个核酸变异位点。除水稻外,所有物种psbL和psbJ基因间区长度均为125bp,水稻psbL和psbJ基因间区为126bp。在psbL和psbJ基因间区,除水稻具有1个插入位点外,还发现8个核酸变异位点。psbL-psbJ区揭示的大麦属物种、黑麦、小麦、水稻和玉米的遗传分化距离变化范围为0.0055-0.0426。以玉米为外类群,采用邻接法进行系统发育关系分析发现,同属于小麦族的大麦属植物、黑麦和小麦间亲缘关系较近,而水稻与玉米则相对较近。

The psbL-psbJ region of chloroplast DNA (cpDNA) of 8 and 2 Hordeum species or subspecies from China and other countries, respectively, were investigated by PCR amplification and direct sequencing. It was indicated that the length of the psbL-psbJ region in all Hordeum species or subspecies was 365 bp, and no variable site was observed. The sequences of psbL-psbJ region were compared with that of rye (Secale cereale L. ) , wheat ( Triticum aestivum L. ) , rice ( Oryza sativa L. ) and maize (Zea mays L. ). The length of psbL gene was 117 bp in all materials, and no variable site was detected. The length of psbJ gene was 123 bp in all materials, and six variable sites were found. The length of psblL-psbJ inter-gene region was 125 bp in all materials except rice, while it was 126 bp in rice. There have 8 variable sites in psbL-psbJ inter-gene region. Using maize as outgroup, phylogenetic relationships were estimated by neighbor-jioning method. It suggested that the species or subspecies of Hordeum were genetically related to rye and wheat, which also belong to the tribe of Triticeae, while rice was related to maize.