间歇及连续式固定化酶反应生产生物柴油

Synthesis of biodiesel using immobilized lipase in batch and continous reactors

探讨了利用本实验室自制的Candidasp99 12 5脂肪酶转酯化合成生物柴油的过程。在利用间歇式反应得到最佳反应条件的情况下利用固定床反应器生产生物柴油,经过初步优化的试验结果表明,在采用分级流加甲醇下,生物柴油的转化率可以达到93%左右,并且固定化酶的使用寿命超过4 80h。

Investigation on the process of production of biodiesel fuel with soybean oil catalyzed by lipase was done in a batch and fixed-bed bioreactor.The optimal reaction condition was obtained in the batch reaction,and the three-step transesterification of beanoil with methanol was conducted in a columns packed with immobilized Candida sp.99-125 lipase.In the process of transesterification, fractional feeding of methanol was conducted to keep the molar ratio of methanol and plantoil as 1∶1, led to the conversion of oil at each step staying above 30% and the total conversion of oil striked 93%.The whole operation was operated continuously .The final conversion of the FAME from waste oil under the optimal condition could reach 92%.The life of the immobilized lipase was more than 20 days.