NGF和FGF对移植的兔5-HT、DA能性神经元的影响

The effects of nerve growth factor and flbroblast growth factor on transplanted 5-HT and DA neu-rons of rabbit.

本实验将含有5—HT和DA能性神经元的胎兔脑组织微块液用立体定向法移植至成年兔脑内。兔21只分为三组,每组7只:第一组加入NGF,第二组加FGF,第三组未加任何因子列为对照组。移植2个月后用高效液相色谱电化学法(HPLC-EC)测定移植神经元的递质(5-HT、DA)含量。结果显示1、2两组的5-HT和第二组DA含量均显著高于对照组(P<0.05,P<0.01)。因此,表明NGF和FGF可能具有促进移植神经元存活和分化的功能。

In the experiment, fetal rabbit brain tissue suspension containing 5,-HT an DA neurons was transplanted into the brain of adult rabbit by stereotaxic method. The animals were divided into 3 groups with 7 each. Nerve growth factor (NGF 3/ig/ml) E.dded in the first group, basic fibroblast growth factor (bFGF 3/ig/ml) in the second group and the third one as control. The neuro -transmitters (5-HT and DA) of transplanted neurons were detected with high performance liquid chromatography-electrical chemistry (HPLC- EC) two months after grafting. The results showed that both 5-HT and DA of group 1 and group 2 increased significantly over group 3 (p<0.05, p<0.01) except DA of group 1. This indicated tha NGF and bFGF can promote survival, differentiation of grafted fetal neurons.