摘要
本文对IL-2基因工程菌的发酵及其包含体制备工艺进行了研究.结果表明在发酵过程中pH的稳定（7.0）、糖的补充（3%）、O2的及时供给（<10PSIG持续泵入）和发酵密度的控制(OD550=1.9),对提高发酵产量至关重要（12g/L）.在包含体制备及溶解工艺中,以4mol/L脲去除脂质及杂蛋白,1%SDS溶解包含体,可获最大IL2活性单位（2.35×107u/ml,即相当于5L发酵可获3.53×109u）.
The study on the technology of fermentation with 5 liter BIOFLO IIc Fermentor (New Brunswick Scientific Co, Inc) and preparation of inclusion body were made. The results showed that the factors including pH stability (7. 0), glucose addition (3%), DO (dissolve oxygen) supplementary (<10 PS1G continuesly added) and controlled cell density (OD500=1.9) played an important role in fermentation. In order to obtain IL-2 of highest activity, 4 mol/L urea was added to remove lipid and cell membranes and 1% SDS was added to dissolve inclusion body. By using this procedure, cell wet weight reached 12 g/L and IL-2 activity increased to 2. 35 107 u/ml (eg. 150 ml×2. 35×10 7 u/ml/5 liter fermentor).
出处
《中国生化药物杂志》
CAS
CSCD
1994年第2期84-86,共3页
Chinese Journal of Biochemical Pharmaceutics
关键词
发酵
包含体
白细胞介素2
重组
IL-2
Fermentation
Inclusion body
Recombinant IL-2
