蚕虫草不同部位营养与活性成分分析

Assays on nutrient and effective ingredients in different parts of Cordyceps militaris

目的:明确蚕虫草不同部位的营养与活性成分,以便开发利用。方法:对蚕虫草、子座、菌核和蚕粉进行分析研究。虫草素和腺苷测定采用高效液相色谱法,虫草多糖测定采用3,5二硝基水杨酸比色法,虫草酸测定采用高碘酸钠比色法,蛋白质测定采用考马氏亮蓝比色法,粗脂肪测定采用索氏提取法。结果:蚕虫草的多糖含量达86.49mg·g-1;子座的腺苷含量达6.82mg·g-1;菌核的虫草素和虫草酸含量分别达到13.28mg·g-1和44.07mg·g-1。结论:不同活性成分在蚕虫草及其不同部位的含量不同,其中蚕虫草活性成分总量最高,菌核虫草素和虫草酸含量最高;研究还表明蛹虫草菌对蚕体脂肪和蛋白质的分解利用能力很强。

Objective: To analysis the nutrient and effective ingredients of in Cordyceps militaris and make the best use of its medical value. Method: Adenosine, cordycepin, polysaccharides, cordyceps acid, protein and fat in different parts of C. militaris were extracted, they are quantified by HPLC and other colorimetric analysis. Result: The contents of polysaccharide was found to be 86.49 mg·g^(-1)in C. militaris, 6.82 mg·g^(-1)of adenosine in stroma, 13.28 mg·g^(-1) of cordycepin and 44.07 mg·g^(-1) of cordyceps acid in sclerolium. Conclusion: In different parts of C. militaris, the biosynthesis of effective ingredients is different. The total amount of effective ingredients is highest in C. militaris, the production of cordycepin and cordyceps acid is highest in sclerotium in comparison with other parts. Growth of C. militaris largely relies on its capability to utilize fat and protein from silkworm.