水解类酶活性在农业废弃物静态高温堆腐过程中的变化

Changes of Some Hydrolase Activities During Agricultural Waste Materials Composting in High Temperature and Static State

在静态通气条件下,以养鸡场鸡粪、小麦秸秆为原料,研究了堆腐过程水解类酶活性、pH值、电导率(EC)变化。结果表明,加入微生物菌剂后纤维素酶、蔗糖酶活性在堆腐过程中的变化趋势同CK处理(不加微生物菌剂)基本一致,但两种酶的活性高峰值都高于CK处理,其峰值分别达到了glucose0.457mg/(g·24h)和glucose87.836mg/(g·24h),两种酶高峰值的出现均早于CK处理,其中纤维素酶活性高峰值较CK处理提前出现2d,蔗糖酶活性高峰值较CK处理提前出现6d;加入微生物菌剂能提高脲酶活性水平及其峰值;加入微生物菌剂处理的pH值相对较低,变化幅度较小;加入微生物菌剂后EC值较高。

An experiment was conducted with mixed materials of chicken excrement and wheat straw to study the changes of some hydrolyses activities, pH value, and election conductance rate (EC) during an aeration composting process in a static state. Results showed that the activities of cellulose enzyme and sucrose enzyme under the condition of adding microorganism fungus had the same tendency in variation with that of no microorganism fungus, the active peak values of the two kinds of enzyme were higher than the treatment of no microorganism fungus, and their peak values were up to 0.457mg glucose/g·24h and 87.836mg glucose/g·24h respectively, the appearances of the two kinds of enzyme peak values were earlier than the treatment of no microorganism fungus, and among them cellulose enzyme was 2d earlier and sucrose enzyme was 6d earlier. Applying microorganism fungus can improve urea enzyme activity and its peak value, reduce the value of pH, and increase the value of EC at the same time.