期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

马立克氏病肿瘤细胞表面抗原的提取与鉴定 被引量：2

Separation and Identification of Marek's Disease Tumor-Associated Surface Antigen
下载PDF
导出
摘要 用MDVGA株感染1日龄SPF雏鸡,饲养至发病后获得MD肿瘤细胞,采用木瓜蛋白酶消化法从MD肿瘤细胞上提取马立克氏病肿瘤相关的表面抗原,经间接荧光抗体染色和间接ELISA鉴定,证明获得的粗提物中含有马立克氏病肿瘤相关的表面抗原,为进行马立克氏病肿瘤相关的表面抗原的进一步研究奠定了基础。 SPF chickens of one day were infected with Marek's disease virus GA and tumor cells were gained from diseased chickens. Marek's disease tumor-associated surface antigen was separated from MD tumor cell by using papain. It was proved that MATSA had been separated from tumor cell by indirect immunofluorescence assay and indirect enzyme linked immunosorbent assay.
作者 穆杨 王玉东 张彦明 童德文 李崴 李玉清 郑增忍
机构地区 西北农林科技大学物科技学院 农业部动物检疫所
出处 《中国农学通报》 CSCD 2005年第5期48-49,103,共3页 Chinese Agricultural Science Bulletin
基金 国家自然科学基金(30371067) 霍英东教育基金(91033)资助。
关键词 马立克氏病 表面抗原 肿瘤细胞 鉴定 提取 间接ELISA 荧光抗体染色 SPF雏鸡 酶消化法 相关 粗提物 MD 日龄 发病 木瓜 MD, Papain, Marek's disease tumor-associated surface antigen, Separate, Identification
分类号 S858.315.3 [农业科学—临床兽医学] Q78 [生物学—分子生物学]
  • 相关文献

参考文献14

  • 1Morimura T, Hattori M, Ohashl Kf, et al. Immumonodulation of peripheral T cells in chickend infected with Marek' s disease virus: involve in ommunosuppression[J]. J Gen Virol,1995,76:2979～2985.
  • 2Witter R L, Stephens E A, Sharma J M, et al. Demonstration of a tumorassociated surface antigen in Marek′s disease [J]. J.Immunol.,1975,115:177～183.
  • 3Ross L J. Characterization of an antigen associated with the Marek′s disease lymphoblsstoid cell line MSB1 [J]. J Gen Virol,60:375～380.
  • 4Nuamnn U, Witerr R L. Differential diagnosis of lymphoid leucosis and Marek′s disease by tumor- associated criteria [J]. Avian Dis, 1979,23:417～433.
  • 5Lesnik F, Ross L J. Immunization against Marek′s disease using Marek′s disease virus-specific antigen from infectious virus[J]. Int J Cancer, 1975,16:153～163.
  • 6Powell P C. Immunity to Marek′s disease induced by glutaraldehyde treated cells of Marek′s disease lymphoblastoid cell lines [J]. Nature, 1975,257:684～685.
  • 7中华人民共和国国家质量监督检验检疫总局.中华人民共和国国家标准GB/T18643-2002(M):鸡马立克氏病诊断技术[M].北京:中国标准出版社,2002.1-4.
  • 8Schat K A, Murthy K K. In vitro cytotoxicity against Marek′s disease lymphoblastoid cell lines after enzymatic removal of Marek′s disease tumorassociated surface antigen[J]. J Virol,1980,34(1):130～135.
  • 9Dandapat S, Pradhan H K, Mohanty G C. Anti-idiotype antibodies to Marek′s disease tumor- associated surface antigen in protection against Marek′s disease[J]. Vet Immunol Immunopathol,1994,40:353～366.
  • 10Matsuda H, Ikuta K, Miyamoto H, et al. Demonstration of a Marek′s disease tumor- associated surface antigen (MATSA) on six cell lines derived from Marek′s disease lymphomas[J]. Biken J, 1976,19(3): 119～23.

同被引文献26

引证文献2

二级引证文献2

中国农学通报
2005年 第5期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部