与大白菜温度敏感胞质雄性不育系育性转化相关的同工酶分析初报

Analysis of Isoenzymes Related with Sterility Changeover in Cytoplasm Male Sterile Line of Chinese Cabbage Sensitived to Temperature

以经过低温处理发生育性转化的大白菜胞质不育系(AT系)、未经处理的不育系(A系)及对应的保持系(B系)6叶苗龄期的根,叶和花期的大、中、小花蕾为试材,采用薄层垂直板聚丙烯酰胺凝胶不连续电泳的方法,分析了POD、ATP、AMY、EST同工酶。结果表明:POD、ATP、AMY同工酶与大白菜不育性没有显著关系;苗期A系与B系酯酶同工酶也无差异,小花蕾差异很大,B系酶活性明显强于A系,且B系比A系多5条α-EST带,其迁移率分别为0.62、0.68、0.72、0.75、0.83等,B系比A系多4条β-EST带,其迁移率分别为0.68、0.72、0.75、0.83。A系经低温处理(9℃下处理18d)发生育性转化后,迁移率为0.83的α-EST带消失,可能与不育性转化有关。结合试验结果还对细胞质雄性不育产生的机理进行了探讨。

The male sterile line of Chinese cabbage with rape cytoplasm, which sterility has been changeover dealt with low temperature(AT line), the male sterile line with no treatment (A line)and it's maintainer (B line ) were used in the experiment. The POD, ATP, AMY,EST isozymes were analyzed with the roots and leaves of seedling stage with six leaves, large buds, middle buds and small buds of flower stage by using vertical slab polyacrylamide gel electrophoresis. The results are shown as follow: POD, ATP, AMY have no notable relations with sterility. EST have no differences in seedling stage between A line and B line, but there are notable differences in small buds (EST's activity of maintainer is stronger than that of sterile line. There are the bands of α-Est with Rf 0.62,0.68,0.72,0.75,0.83 in maintainer more than that of sterile line and the bands of β-Est with Rf 0.68,0.72,0.75,0.83 of maintainer more than that of sterile line).The band of α-Est with Rf 0.83 was disappeared with the changeover of sterility by the treatment of low temperature, which may be related with the changeover of sterility. Based on the experiment the mechanisms of cytoplasm male sterility are also discussed in this paper.