红细胞聚集的电导法研究

STUDY OF RBC AGGREGATION WITH CONDUCTIVE METHOD

依据Ｓ．Ｖｅｌｉｃｈ提出的随机取向红细胞的悬浮液电导方程，提出了测量红细胞聚集动态过程的电导法。在仪器的设计中采用四电极方案克服血液中由于蛋白质等物质吸附在电极表面上对测量结果所造成的影响，采用载波技术克服电极极化所造成的影响，从而提高了测量系统的信噪比、灵敏度和可靠性。根据红细胞悬浮液的电导理论，在实验数据的基础上，建立了描述红细胞聚集的动态过程方程：Ｉ＝Ｉ_ｓ－（Ｉ_ｓ－Ｉ_ｍ）·ｅｘＰ（－（ｔ_ｍ－ｔ）／τ），从而得到了用该法描述红细胞聚集程度指标：聚集指数ＡＩ＝Ｉ_ｓ－Ｉ_ｍ，描述红细胞聚集动态过程的参数：时间常数τ。

Acording to the equation of blood conductivity for RBC of random orientation; H=(Io/I-1)/(Io/I-1+f),a new conductive method was presented for measuring kinetic process of RBC aggregation.The whole measuring system consisted of four parts:conductive cell,four-electrodes conductive gauge,computer system and water thermostat.In the designing of four-electrodes conductive gauge,we adopted four-electrodes system to overcome electrode pollution,and adopted carrier technique to overcome electrode polarization.So the signal-noise ratio,sensitivity and reliability of the measuring system were greatly enhanced.Based on experimetal data and conductive theory of RBC suspension equation describing kinetic process of RBC rouleau formation was introduced:I(t)=I_s-(I_s-I_m)·e￣-(t_m-t)/τAnd hence an aggregation index AI(Is-Im) and a time constant τwere also obtained as index describing RBC kinetic aggregation.This equation well accorded with experimental data.